immunology & Biochemistry
Nafise Annabestani; Hamid Mollania; Nasrin Mollania
Abstract
Introduction: Herbal medicine, which bears fewer adverse effects than chemical medications, is becoming more popular for the treatment of diabetes.Materials and Methods: After measuring the antioxidant properties, the inhibition pattern between the extracts and the pancreatic α-amylase enzyme was ...
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Introduction: Herbal medicine, which bears fewer adverse effects than chemical medications, is becoming more popular for the treatment of diabetes.Materials and Methods: After measuring the antioxidant properties, the inhibition pattern between the extracts and the pancreatic α-amylase enzyme was determined. Diabetic mice were treated with herbal extracts and acarbose. The blood biochemical factors were then measured. The best extract examined the synthesis of silver nanoparticles (Ag-NPs).Results: The extracts of saffron and chamomile petals showed an inhibitory effect on the α-amylase enzyme with a competitive and mixed pattern, respectively. FBS had a significant decrease in all treatment groups, and only in the group treated with an ultrasound-assisted aqueous extract of saffron petals did insulin levels increase significantly. The ALT enzyme decreased significantly in the presence of saffron extract. The reduction of the AST factor is significant only in chamomile extract. The results showed that for optimal synthesis of silver nanoparticles, values of temperature, pH, time, and silver nitrate salt concentration were 55°C, pH = 10, 4 hours, and 1.2 mM, respectively.Conclusion: The plant extracts studies can efficiently lower the blood sugar levels, increase insulin production, and regulate liver enzymes in the blood. As a result, they have a good impact on diabetes treatment. These extracts outperform the chemical medication acarbose in terms of efficacy and have no negative effects on the liver. The saffron extract demonstrates the ability to synthesize Ag-NP, which is frequently employed in biomedical applications.
Nasrin Mollania; Farangis Gharibnia; Ramin Rostami- Taghi Dizaj; Mitra Kheyrabadi
Volume 23, Issue 2 , March and April 2016, , Pages 214-221
Abstract
Background: With the advent and increasing microbial organisms that resistant to multiple antibiotics, as well as the necessity of decrease the cost of health care, the production of broad range of anti-microbial materials has become unavoidable for human societies. In this study, we synthesized the ...
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Background: With the advent and increasing microbial organisms that resistant to multiple antibiotics, as well as the necessity of decrease the cost of health care, the production of broad range of anti-microbial materials has become unavoidable for human societies. In this study, we synthesized the silver nanoparticles using bacterial α-amylase enzyme and evaluated the antibacterial properties of this eco-friendly nanoparticles. Methods: Silver nanoparticles were synthesized biologically using bacterial α-amylase enzyme, then the effects of antibacterial nanoparticles synthesized in LB medium containing various strains of pathogenic bacteria were investigated and then the minimal inhibitory concentration was calculated. Results: Nanoparticles produced by this method, based on DLS and SEM analysis, have the size of 20-40 nm and have good activity against Gram-positive bacteria and gram negative pathogenic bacteria. In the 200 of Ag-NPs, the all of pathogenic bacteria was killed. Conclusion: Biological nanoparticles synthesis relative to other physical and chemical methods has lower cost and higher durability (1). Due to good antibacterial activity of silver nanoparticles, this material has good medical applications.